Rapid detection of influenza A neuraminidase subtypes by cDNA amplification coupled to a simple DNA enzyme immunoassay
Identifieur interne : 001E22 ( Main/Exploration ); précédent : 001E21; suivant : 001E23Rapid detection of influenza A neuraminidase subtypes by cDNA amplification coupled to a simple DNA enzyme immunoassay
Auteurs : B. Schweiger [Allemagne] ; I. Lange [Allemagne] ; R. Heckler [Allemagne] ; H. Willers [Allemagne] ; E. Schreier [Allemagne]Source :
- Archives of Virology [ 0304-8608 ] ; 1994-09-01.
Descripteurs français
- KwdFr :
- ADN complémentaire (analyse), ADN complémentaire (génétique), Données de séquences moléculaires, Génome viral, Hybridation d'acides nucléiques, Réaction de polymérisation en chaîne, Sensibilité et spécificité, Sialidase (), Sialidase (génétique), Sondes d'ADN, Séquence nucléotidique, Techniques immunoenzymatiques, Virus de la grippe A (), Virus de la grippe A (enzymologie), Virus de la grippe A (génétique).
- MESH :
- analyse : ADN complémentaire.
- enzymologie : Virus de la grippe A.
- génétique : ADN complémentaire, Sialidase, Virus de la grippe A.
- Pascal (Inist)
- Anticorps monoclonal, Données de séquences moléculaires, Détection, Exo-α-sialidase, Génome viral, Hybridation d'acides nucléiques, Hybridation moléculaire, Influenzavirus A, Méthode, Méthode immunoenzymatique, Réaction chaîne polymérase, Réaction de polymérisation en chaîne, Sensibilité et spécificité, Sialidase, Sondes d'ADN, Spécificité type, Séquence nucléotidique, Technique ELISA, Techniques immunoenzymatiques, Virus de la grippe A.
English descriptors
- KwdEn :
- Base Sequence, DNA Probes, DNA, Complementary (analysis), DNA, Complementary (genetics), Detection, ELISA assay, Enzyme immunoassay, Exo-α-sialidase, Genome, Viral, Immunoenzyme Techniques, Influenza A virus (classification), Influenza A virus (enzymology), Influenza A virus (genetics), Influenzavirus A, Method, Molecular Sequence Data, Molecular hybridization, Monoclonal antibody, Neuraminidase (classification), Neuraminidase (genetics), Nucleic Acid Hybridization, Polymerase Chain Reaction, Polymerase chain reaction, Sensitivity and Specificity, Type specificity.
- MESH :
- chemical , analysis : DNA, Complementary.
- chemical , classification : Neuraminidase.
- chemical , genetics : DNA, Complementary, Neuraminidase.
- chemical : DNA Probes.
- classification : Influenza A virus.
- enzymology : Influenza A virus.
- genetics : Influenza A virus.
- Base Sequence, Genome, Viral, Immunoenzyme Techniques, Molecular Sequence Data, Nucleic Acid Hybridization, Polymerase Chain Reaction, Sensitivity and Specificity.
- Teeft :
- Amplification, Arch virol, Assay, Cdna, Cdna amplification, Chemiluminescent detection, Complete nucleotide sequence, Deia, Embryonated eggs, Enzyme immunoassay, Ethidium bromide, Federal republic, Human influenza, Human influenza virus, Hybridization, Hybridization procedures, Immunoassay, Influenza, Influenza virus, Influenza virus neuraminidase gene, Influenza viruses, Large number, Microtiter, Microtiter wells, Neuraminidase, Neuraminidase gene, Neuraminidase subtypes, Optical density, Polymerase, Polymerase chain reaction, Primer, Rapid detection, Rapid diagnosis, Respiratory secretions, Schreier, Schweiger, Specific probe, Subtypes, Subtyping, Uninfected allantoic fluid, Universal primers, Virol, Virol methods, Virology, Virus strains.
Abstract
Summary: A newly developed colorimetric method, DNA enzyme immunoassay (DEIA), was applied to the detection of neuraminidase subtypes N1 and N2 of influenza A viruses. Reverse transcription and polymerase chain reaction with universal primers were used for genomic amplification of H1N1, H2N2, and H3N2 strains. Following amplification, an aliquot of the PCR product was hybridized to biotinylated DNA sequences (N1/N2 probes) immobilized on microtiter wells. The hybridization event was revealed by monoclonal antibodies to double stranded DNA in a standard ELISA reaction. The assay described here was able to distinguish accurately between the two neuraminidase subtypes of human influenza A viruses. It is a simple and rapid method facilitating the handling of a large number of samples and therefore seems to be easily applicable to diagnostic laboratories.
Url:
DOI: 10.1007/BF01310805
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000026
- to stream Istex, to step Curation: 000026
- to stream Istex, to step Checkpoint: 000B93
- to stream Main, to step Merge: 001F04
- to stream PascalFrancis, to step Corpus: 000092
- to stream PascalFrancis, to step Curation: 000122
- to stream PascalFrancis, to step Checkpoint: 000095
- to stream Main, to step Merge: 001F32
- to stream PubMed, to step Corpus: 000419
- to stream PubMed, to step Curation: 000419
- to stream PubMed, to step Checkpoint: 000378
- to stream Ncbi, to step Merge: 001298
- to stream Ncbi, to step Curation: 001298
- to stream Ncbi, to step Checkpoint: 001298
- to stream Main, to step Merge: 001E56
- to stream Main, to step Curation: 001E22
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid detection of influenza A neuraminidase subtypes by cDNA amplification coupled to a simple DNA enzyme immunoassay</title>
<author><name sortKey="Schweiger, B" sort="Schweiger, B" uniqKey="Schweiger B" first="B." last="Schweiger">B. Schweiger</name>
</author>
<author><name sortKey="Lange, I" sort="Lange, I" uniqKey="Lange I" first="I." last="Lange">I. Lange</name>
</author>
<author><name sortKey="Heckler, R" sort="Heckler, R" uniqKey="Heckler R" first="R." last="Heckler">R. Heckler</name>
</author>
<author><name sortKey="Willers, H" sort="Willers, H" uniqKey="Willers H" first="H." last="Willers">H. Willers</name>
</author>
<author><name sortKey="Schreier, E" sort="Schreier, E" uniqKey="Schreier E" first="E." last="Schreier">E. Schreier</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:05CE0F93E29A6B9FE384C21EA8C518535E8921EF</idno>
<date when="1994" year="1994">1994</date>
<idno type="doi">10.1007/BF01310805</idno>
<idno type="url">https://api.istex.fr/ark:/67375/1BB-TQ545LHC-4/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000026</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000026</idno>
<idno type="wicri:Area/Istex/Curation">000026</idno>
<idno type="wicri:Area/Istex/Checkpoint">000B93</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000B93</idno>
<idno type="wicri:doubleKey">0304-8608:1994:Schweiger B:rapid:detection:of</idno>
<idno type="wicri:Area/Main/Merge">001F04</idno>
<idno type="wicri:source">INIST</idno>
<idno type="RBID">Pascal:95-0082268</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000092</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000122</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000095</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000095</idno>
<idno type="wicri:doubleKey">0304-8608:1994:Schweiger B:rapid:detection:of</idno>
<idno type="wicri:Area/Main/Merge">001F32</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:7832649</idno>
<idno type="wicri:Area/PubMed/Corpus">000419</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000419</idno>
<idno type="wicri:Area/PubMed/Curation">000419</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000419</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000378</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000378</idno>
<idno type="wicri:Area/Ncbi/Merge">001298</idno>
<idno type="wicri:Area/Ncbi/Curation">001298</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001298</idno>
<idno type="wicri:doubleKey">0304-8608:1994:Schweiger B:rapid:detection:of</idno>
<idno type="wicri:Area/Main/Merge">001E56</idno>
<idno type="wicri:Area/Main/Curation">001E22</idno>
<idno type="wicri:Area/Main/Exploration">001E22</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Rapid detection of influenza A neuraminidase subtypes by cDNA amplification coupled to a simple DNA enzyme immunoassay</title>
<author><name sortKey="Schweiger, B" sort="Schweiger, B" uniqKey="Schweiger B" first="B." last="Schweiger">B. Schweiger</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Robert Koch-Institut, Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lange, I" sort="Lange, I" uniqKey="Lange I" first="I." last="Lange">I. Lange</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Robert Koch-Institut, Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Heckler, R" sort="Heckler, R" uniqKey="Heckler R" first="R." last="Heckler">R. Heckler</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Staatliches Medizinaluntersuchungsamt, Hannover</wicri:regionArea>
<placeName><region type="land" nuts="2">Basse-Saxe</region>
<settlement type="city">Hanovre</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Willers, H" sort="Willers, H" uniqKey="Willers H" first="H." last="Willers">H. Willers</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Staatliches Medizinaluntersuchungsamt, Hannover</wicri:regionArea>
<placeName><region type="land" nuts="2">Basse-Saxe</region>
<settlement type="city">Hanovre</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Schreier, E" sort="Schreier, E" uniqKey="Schreier E" first="E." last="Schreier">E. Schreier</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Robert Koch-Institut, Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Archives of Virology</title>
<title level="j" type="abbrev">Archives of Virology</title>
<idno type="ISSN">0304-8608</idno>
<idno type="eISSN">1432-8798</idno>
<imprint><publisher>Springer-Verlag</publisher>
<pubPlace>Vienna</pubPlace>
<date type="published" when="1994-09-01">1994-09-01</date>
<biblScope unit="volume">139</biblScope>
<biblScope unit="issue">3-4</biblScope>
<biblScope unit="page" from="439">439</biblScope>
<biblScope unit="page" to="444">444</biblScope>
</imprint>
<idno type="ISSN">0304-8608</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0304-8608</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term>
<term>DNA Probes</term>
<term>DNA, Complementary (analysis)</term>
<term>DNA, Complementary (genetics)</term>
<term>Detection</term>
<term>ELISA assay</term>
<term>Enzyme immunoassay</term>
<term>Exo-α-sialidase</term>
<term>Genome, Viral</term>
<term>Immunoenzyme Techniques</term>
<term>Influenza A virus (classification)</term>
<term>Influenza A virus (enzymology)</term>
<term>Influenza A virus (genetics)</term>
<term>Influenzavirus A</term>
<term>Method</term>
<term>Molecular Sequence Data</term>
<term>Molecular hybridization</term>
<term>Monoclonal antibody</term>
<term>Neuraminidase (classification)</term>
<term>Neuraminidase (genetics)</term>
<term>Nucleic Acid Hybridization</term>
<term>Polymerase Chain Reaction</term>
<term>Polymerase chain reaction</term>
<term>Sensitivity and Specificity</term>
<term>Type specificity</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (analyse)</term>
<term>ADN complémentaire (génétique)</term>
<term>Données de séquences moléculaires</term>
<term>Génome viral</term>
<term>Hybridation d'acides nucléiques</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sensibilité et spécificité</term>
<term>Sialidase ()</term>
<term>Sialidase (génétique)</term>
<term>Sondes d'ADN</term>
<term>Séquence nucléotidique</term>
<term>Techniques immunoenzymatiques</term>
<term>Virus de la grippe A ()</term>
<term>Virus de la grippe A (enzymologie)</term>
<term>Virus de la grippe A (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Complementary</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Neuraminidase</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term>
<term>Neuraminidase</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Probes</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN complémentaire</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Influenza A virus</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Influenza A virus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term>
<term>Sialidase</term>
<term>Virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Genome, Viral</term>
<term>Immunoenzyme Techniques</term>
<term>Molecular Sequence Data</term>
<term>Nucleic Acid Hybridization</term>
<term>Polymerase Chain Reaction</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Anticorps monoclonal</term>
<term>Données de séquences moléculaires</term>
<term>Détection</term>
<term>Exo-α-sialidase</term>
<term>Génome viral</term>
<term>Hybridation d'acides nucléiques</term>
<term>Hybridation moléculaire</term>
<term>Influenzavirus A</term>
<term>Méthode</term>
<term>Méthode immunoenzymatique</term>
<term>Réaction chaîne polymérase</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sensibilité et spécificité</term>
<term>Sialidase</term>
<term>Sondes d'ADN</term>
<term>Spécificité type</term>
<term>Séquence nucléotidique</term>
<term>Technique ELISA</term>
<term>Techniques immunoenzymatiques</term>
<term>Virus de la grippe A</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Amplification</term>
<term>Arch virol</term>
<term>Assay</term>
<term>Cdna</term>
<term>Cdna amplification</term>
<term>Chemiluminescent detection</term>
<term>Complete nucleotide sequence</term>
<term>Deia</term>
<term>Embryonated eggs</term>
<term>Enzyme immunoassay</term>
<term>Ethidium bromide</term>
<term>Federal republic</term>
<term>Human influenza</term>
<term>Human influenza virus</term>
<term>Hybridization</term>
<term>Hybridization procedures</term>
<term>Immunoassay</term>
<term>Influenza</term>
<term>Influenza virus</term>
<term>Influenza virus neuraminidase gene</term>
<term>Influenza viruses</term>
<term>Large number</term>
<term>Microtiter</term>
<term>Microtiter wells</term>
<term>Neuraminidase</term>
<term>Neuraminidase gene</term>
<term>Neuraminidase subtypes</term>
<term>Optical density</term>
<term>Polymerase</term>
<term>Polymerase chain reaction</term>
<term>Primer</term>
<term>Rapid detection</term>
<term>Rapid diagnosis</term>
<term>Respiratory secretions</term>
<term>Schreier</term>
<term>Schweiger</term>
<term>Specific probe</term>
<term>Subtypes</term>
<term>Subtyping</term>
<term>Uninfected allantoic fluid</term>
<term>Universal primers</term>
<term>Virol</term>
<term>Virol methods</term>
<term>Virology</term>
<term>Virus strains</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Summary: A newly developed colorimetric method, DNA enzyme immunoassay (DEIA), was applied to the detection of neuraminidase subtypes N1 and N2 of influenza A viruses. Reverse transcription and polymerase chain reaction with universal primers were used for genomic amplification of H1N1, H2N2, and H3N2 strains. Following amplification, an aliquot of the PCR product was hybridized to biotinylated DNA sequences (N1/N2 probes) immobilized on microtiter wells. The hybridization event was revealed by monoclonal antibodies to double stranded DNA in a standard ELISA reaction. The assay described here was able to distinguish accurately between the two neuraminidase subtypes of human influenza A viruses. It is a simple and rapid method facilitating the handling of a large number of samples and therefore seems to be easily applicable to diagnostic laboratories.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
</country>
<region><li>Basse-Saxe</li>
<li>Berlin</li>
</region>
<settlement><li>Berlin</li>
<li>Hanovre</li>
</settlement>
</list>
<tree><country name="Allemagne"><region name="Berlin"><name sortKey="Schweiger, B" sort="Schweiger, B" uniqKey="Schweiger B" first="B." last="Schweiger">B. Schweiger</name>
</region>
<name sortKey="Heckler, R" sort="Heckler, R" uniqKey="Heckler R" first="R." last="Heckler">R. Heckler</name>
<name sortKey="Lange, I" sort="Lange, I" uniqKey="Lange I" first="I." last="Lange">I. Lange</name>
<name sortKey="Schreier, E" sort="Schreier, E" uniqKey="Schreier E" first="E." last="Schreier">E. Schreier</name>
<name sortKey="Willers, H" sort="Willers, H" uniqKey="Willers H" first="H." last="Willers">H. Willers</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E22 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001E22 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:05CE0F93E29A6B9FE384C21EA8C518535E8921EF |texte= Rapid detection of influenza A neuraminidase subtypes by cDNA amplification coupled to a simple DNA enzyme immunoassay }}
This area was generated with Dilib version V0.6.33. |